Development of nano-coated granules containing trans-resveratrol

Abstract

The present study developed and characterized trans-resveratrol (RSV) nano-coated granules. Thus, polymeric nanocapsules containing RSV (NC-RSV, 0.5 mg/mL) and blank nanocapsules (NCB) were prepared by interfacial deposition of preformed polymer. The formulations were characterized for diameter, zeta potential, pH and drug content. The cytotoxicity of NC-RSV was assessed by the MTT technique in macrophages (RAW strain) at concentrations of 0.78 – 100 ?g/mL for 24, 48 and 72 h. NCB, free RSV and Tween 80 were also tested for comparison. Next, wet granules were prepared using HPMC or PVP as binder, which were characterized for drug content, flow properties and compaction capacity. The NC-RSV had an average size of 188±3 nm, zeta potential -26.5±0.2 mV, pH 5.4 and a mean drug content of 0.4904±0.0056 mg/mL. In the MTT test, cells exposed to NC-RSV presented cell viability greater than 70% at concentrations of 0.78 to 6.25 ?g/mL. The HPMC agglutinated granules showed a yield of 37.9±4.5% for RSV and all powders showed fair and passable flow properties. In this way, the use of nanotechnology offers a promising drug delivery system and the incorporation of the nanoformulations into granules allows the design of pharmaceutical forms with better stability, although other studies are necessary to adjust the excipients to obtain more adequate preparations.

Keywords: trans-resveratrol. Nanotechnology. Cytotoxicity. Granules.